Pathological, immunohistochemical, and molecular findings in commercial laying hens and in backyard chickens naturally infected with the infectious laryngotracheitis virus

Detection of Infectious Laryngotracheitis Virus by Real-Time PCR in Naturally and Experimentally Infected Chickens

Infectious laryngotracheitis (ILT) is an acute, highly contagious upper-respiratory infectious disease of chickens. In this study, a real-time PCR method was developed for fast and accurate detection and quantitation of ILTV DNA of chickens experimentally infected with ILTV strain LJS09 and naturally infected chickens. The detection lower limit of the assay was 10 copies of DNA. There were no c...

Pathological, Immunohistochemical, and Electron Microscopic Findings in the Respiratory Tract and Skin of Chickens Naturally Infected with Avipoxvirus

The present study describes pathological, immunohistochemical, and electron microscopic findings in the respiratory tract and skin of chickens naturally infected with avian pox virus. Respiratory distress, depression, and cyanosis were found in the chickens upon clinical examination. At necropsy, erosion, crusts, and nodules were observed on the comb, wattle, and eyelids. Histopathological exam...

Isolation and Identification of Infectious Bronchitis Virus from Commercial Chickens

Comparison of clinico - pathological changes in SPF chickens infected with different Iranian genotypes of infectious bronchitis virus

Introduction: Infectious bronchitis (IB), caused by infectious bronchitis virus (IBV), is an acute and highly contagious disease in chickens. IBV is mainly considered a respiratory infection, other clinical manifestations, including renal, enteric and reproductive signs can be observed. Aim: Since there has been no study on evaluation of changes in biochemical parameters during IB infection, th...

Detection of infectious laryngotracheitis virus (Gallid herpesvirus-1) from clinically infected chickens in Egypt by different diagnostic methods

Infectious laryngotracheitis (ILT) disease is an acute highly contagious viral disease leading to massive economic losses to the national poultry industry. This study aimed to identify the most accurate and rapid diagnostic methods to rescue layer poultry farms from intense outbreaks in Egypt. Fifty pathological specimens were collected and subjected to virus isolation (VI), histopathology, dir...